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gelredtm nucleic acid stain  (Biotium)


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    Biotium gelredtm nucleic acid stain
    Gelredtm Nucleic Acid Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gelredtm+nucleic+acid+stain/GelRed+Nucleic+Acid+Gel+Stain/pm42123583-205-4-10
    Average 97 stars, based on 3998 article reviews
    gelredtm nucleic acid stain - by Bioz Stars, 2026-11
    97/100 stars

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    other:

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRed Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE).

    Staining:

    Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential
    Article Snippet: Trypsin–EDTA 0.25% and fetal bovine serum (FBS) were purchased from HyClone, Thermo Scientific (Logan, UT, USA). .. Agarose and GelRedTM nucleic acid stain were acquired from Biotium (Fremont, CA, USA). .. Restriction enzymes XbaI and BlpI were obtained from New England Biolabs (Ipswich, MA, USA).

    Article Title: Photoperiod-Mediated Transcriptomic Regulation of Spawning in Octopus mimus
    Article Snippet: Total RNA was extracted from each tissue sample using the NZY Total RNA Isolation Kit (NZYtech, Lisbon, Portugal) following the manufacturer’s protocol. .. RNA concentration was measured with DeNovix DS-11 FS and RNA integrity was checked by running an aliquot of total RNA (~500 ng) on a 1% (w/v) agarose gel stained with GelRedTM nucleic acid stain (Biotium, Hayward, CA, USA). .. The RNA quality was evaluated by sample absorbance according to the A260/280 and A260/230 ratios using a BiotTek® Synergy HT microplate reader (Synergy HT, BioTek Instruments, Inc., Winooski, VT, USA).

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce (Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% (w/v) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers (Table S4), in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 ◦C for 30 s, followed by 39 cycles of 95 ◦C for 10 s, 59 ◦C for 10 s and 72 ◦C for 30 s. The relative steady-state transcript abundance was quantified using the 2−∆∆CT method [48].

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce ( Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% ( w / v ) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers , in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 °C for 30 s, followed by 39 cycles of 95 °C for 10 s, 59 °C for 10 s and 72 °C for 30 s. The relative steady-state transcript abundance was quantified using the 2 −ΔΔCT method [ ].

    Article Title: Serovars, Genetic Relatedness and Antimicrobial Resistance of Non-Typhoidal Salmonella in Poultry and Farm Workers in Southeastern Nigeria
    Article Snippet: Each 25 μL PCR contained 1 μL each of forward and reverse ERIC primers (20 pmol/L; Inqaba Biotechnical Industries, Pretoria, South Africa) , 2.5 μL of 10× PCR buffer, 2.0 μL of 2.5 mmol/L dNTPs, 1.5 μL of 25 mmol/L MgCl 2 , 5 U of Taq DNA polymerase, and 1.5 μL of genomic DNA. .. Conventional PCR cycling conditions consisted of an initial denaturation at 94 °C for 10 min, followed by 31 cycles of denaturation at 92 °C for 30 s, annealing at 40 °C for 60 s, and extension at 65 °C for 8 min, with a final extension at 65 °C for 8 min. Amplified products (5 μL) were mixed with GelRedTM nucleic acid stain (1:500 dilution) (Biotium Incorporated, Fremont, CA, USA) and resolved on 1.5% agarose gels at 100 V for 1 h. Gels were visualized under ultraviolet light using a Bio-Rad gel imaging system (Bio-Rad, CA, USA). ..

    Article Title: Evaluation of Root Films with Bacillus subtilis for Establishment and Growth Promotion in Tomato.
    Article Snippet: .. The presence of DNA was verified by electrophoresis on 1% (w/v) agarose gels prepared in 1× TAE buffer and run at 90 V for 30 min. DNA bands were visualized under UV light using a BIORAD Universal Hood II (USA) photodocumenter after staining with GelRedTM nucleic acid stain (Biotium, Fremont, CA, USA). ..

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: .. Samples were visualized by GelRedTM Nucleic Acid Stain (cat 41003, Biotium, Fremont, CA, USA) using the Azure 300 imaging system (cat AZI30001, Azure Biosystems, Dublin, CA, USA). .. The image quantification was performed by densitometric analysis using Image Lab software v6.1 (cat 12012931, Bio-Rad Laboratories, Hercules, CA, USA).

    Concentration Assay:

    Article Title: Photoperiod-Mediated Transcriptomic Regulation of Spawning in Octopus mimus
    Article Snippet: Total RNA was extracted from each tissue sample using the NZY Total RNA Isolation Kit (NZYtech, Lisbon, Portugal) following the manufacturer’s protocol. .. RNA concentration was measured with DeNovix DS-11 FS and RNA integrity was checked by running an aliquot of total RNA (~500 ng) on a 1% (w/v) agarose gel stained with GelRedTM nucleic acid stain (Biotium, Hayward, CA, USA). .. The RNA quality was evaluated by sample absorbance according to the A260/280 and A260/230 ratios using a BiotTek® Synergy HT microplate reader (Synergy HT, BioTek Instruments, Inc., Winooski, VT, USA).

    Agarose Gel Electrophoresis:

    Article Title: Photoperiod-Mediated Transcriptomic Regulation of Spawning in Octopus mimus
    Article Snippet: Total RNA was extracted from each tissue sample using the NZY Total RNA Isolation Kit (NZYtech, Lisbon, Portugal) following the manufacturer’s protocol. .. RNA concentration was measured with DeNovix DS-11 FS and RNA integrity was checked by running an aliquot of total RNA (~500 ng) on a 1% (w/v) agarose gel stained with GelRedTM nucleic acid stain (Biotium, Hayward, CA, USA). .. The RNA quality was evaluated by sample absorbance according to the A260/280 and A260/230 ratios using a BiotTek® Synergy HT microplate reader (Synergy HT, BioTek Instruments, Inc., Winooski, VT, USA).

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce (Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% (w/v) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers (Table S4), in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 ◦C for 30 s, followed by 39 cycles of 95 ◦C for 10 s, 59 ◦C for 10 s and 72 ◦C for 30 s. The relative steady-state transcript abundance was quantified using the 2−∆∆CT method [48].

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce ( Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% ( w / v ) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers , in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 °C for 30 s, followed by 39 cycles of 95 °C for 10 s, 59 °C for 10 s and 72 °C for 30 s. The relative steady-state transcript abundance was quantified using the 2 −ΔΔCT method [ ].

    Polymerase Chain Reaction:

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce (Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% (w/v) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers (Table S4), in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 ◦C for 30 s, followed by 39 cycles of 95 ◦C for 10 s, 59 ◦C for 10 s and 72 ◦C for 30 s. The relative steady-state transcript abundance was quantified using the 2−∆∆CT method [48].

    Article Title: Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce ( Lactuca sativa L. cv. Salinas)
    Article Snippet: .. The PCR products were visualized on a 1.5% ( w / v ) agarose gel after staining with GelRedTM Nucleic Acid Stain (Biotium, Fremont, CA, USA). .. Quantitative real-time PCRs (qRT-PCRs) were performed using iTaq Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) with gene-specific primers , in a CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with the following cycles: 95 °C for 30 s, followed by 39 cycles of 95 °C for 10 s, 59 °C for 10 s and 72 °C for 30 s. The relative steady-state transcript abundance was quantified using the 2 −ΔΔCT method [ ].

    Article Title: Serovars, Genetic Relatedness and Antimicrobial Resistance of Non-Typhoidal Salmonella in Poultry and Farm Workers in Southeastern Nigeria
    Article Snippet: Each 25 μL PCR contained 1 μL each of forward and reverse ERIC primers (20 pmol/L; Inqaba Biotechnical Industries, Pretoria, South Africa) , 2.5 μL of 10× PCR buffer, 2.0 μL of 2.5 mmol/L dNTPs, 1.5 μL of 25 mmol/L MgCl 2 , 5 U of Taq DNA polymerase, and 1.5 μL of genomic DNA. .. Conventional PCR cycling conditions consisted of an initial denaturation at 94 °C for 10 min, followed by 31 cycles of denaturation at 92 °C for 30 s, annealing at 40 °C for 60 s, and extension at 65 °C for 8 min, with a final extension at 65 °C for 8 min. Amplified products (5 μL) were mixed with GelRedTM nucleic acid stain (1:500 dilution) (Biotium Incorporated, Fremont, CA, USA) and resolved on 1.5% agarose gels at 100 V for 1 h. Gels were visualized under ultraviolet light using a Bio-Rad gel imaging system (Bio-Rad, CA, USA). ..

    Amplification:

    Article Title: Serovars, Genetic Relatedness and Antimicrobial Resistance of Non-Typhoidal Salmonella in Poultry and Farm Workers in Southeastern Nigeria
    Article Snippet: Each 25 μL PCR contained 1 μL each of forward and reverse ERIC primers (20 pmol/L; Inqaba Biotechnical Industries, Pretoria, South Africa) , 2.5 μL of 10× PCR buffer, 2.0 μL of 2.5 mmol/L dNTPs, 1.5 μL of 25 mmol/L MgCl 2 , 5 U of Taq DNA polymerase, and 1.5 μL of genomic DNA. .. Conventional PCR cycling conditions consisted of an initial denaturation at 94 °C for 10 min, followed by 31 cycles of denaturation at 92 °C for 30 s, annealing at 40 °C for 60 s, and extension at 65 °C for 8 min, with a final extension at 65 °C for 8 min. Amplified products (5 μL) were mixed with GelRedTM nucleic acid stain (1:500 dilution) (Biotium Incorporated, Fremont, CA, USA) and resolved on 1.5% agarose gels at 100 V for 1 h. Gels were visualized under ultraviolet light using a Bio-Rad gel imaging system (Bio-Rad, CA, USA). ..

    Imaging:

    Article Title: Serovars, Genetic Relatedness and Antimicrobial Resistance of Non-Typhoidal Salmonella in Poultry and Farm Workers in Southeastern Nigeria
    Article Snippet: Each 25 μL PCR contained 1 μL each of forward and reverse ERIC primers (20 pmol/L; Inqaba Biotechnical Industries, Pretoria, South Africa) , 2.5 μL of 10× PCR buffer, 2.0 μL of 2.5 mmol/L dNTPs, 1.5 μL of 25 mmol/L MgCl 2 , 5 U of Taq DNA polymerase, and 1.5 μL of genomic DNA. .. Conventional PCR cycling conditions consisted of an initial denaturation at 94 °C for 10 min, followed by 31 cycles of denaturation at 92 °C for 30 s, annealing at 40 °C for 60 s, and extension at 65 °C for 8 min, with a final extension at 65 °C for 8 min. Amplified products (5 μL) were mixed with GelRedTM nucleic acid stain (1:500 dilution) (Biotium Incorporated, Fremont, CA, USA) and resolved on 1.5% agarose gels at 100 V for 1 h. Gels were visualized under ultraviolet light using a Bio-Rad gel imaging system (Bio-Rad, CA, USA). ..

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: .. Samples were visualized by GelRedTM Nucleic Acid Stain (cat 41003, Biotium, Fremont, CA, USA) using the Azure 300 imaging system (cat AZI30001, Azure Biosystems, Dublin, CA, USA). .. The image quantification was performed by densitometric analysis using Image Lab software v6.1 (cat 12012931, Bio-Rad Laboratories, Hercules, CA, USA).

    Electrophoresis:

    Article Title: Evaluation of Root Films with Bacillus subtilis for Establishment and Growth Promotion in Tomato.
    Article Snippet: .. The presence of DNA was verified by electrophoresis on 1% (w/v) agarose gels prepared in 1× TAE buffer and run at 90 V for 30 min. DNA bands were visualized under UV light using a BIORAD Universal Hood II (USA) photodocumenter after staining with GelRedTM nucleic acid stain (Biotium, Fremont, CA, USA). ..

    Hood:

    Article Title: Evaluation of Root Films with Bacillus subtilis for Establishment and Growth Promotion in Tomato.
    Article Snippet: .. The presence of DNA was verified by electrophoresis on 1% (w/v) agarose gels prepared in 1× TAE buffer and run at 90 V for 30 min. DNA bands were visualized under UV light using a BIORAD Universal Hood II (USA) photodocumenter after staining with GelRedTM nucleic acid stain (Biotium, Fremont, CA, USA). ..



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